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. 1997 Nov 17;186(10):1713–1724. doi: 10.1084/jem.186.10.1713

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Effects of F505 Lck and cellular stimulation on the kinetics of plasma membrane acquisition of ZAP-70 GFP and KD ZAP-70 GFP. Individual live Cos 7 cells were examined by confocal microscopy and plasma membrane acquisition of ZAP-70 GFP assessed as described in Materials and Methods. A single image of each cell is shown before activation (unstim) with subsequent images shown at 1-min intervals after the addition of PV. Arrows highlight specific areas of plasma membrane accumulation of the ZAP-70 GFP fusion proteins.