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. 1997 Nov 17;186(10):1713–1724. doi: 10.1084/jem.186.10.1713

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ZAP-70 GFP expression and cellular location in Cos 7 cells. Cos 7 cells expressing pEGFP/ZAP-70 alone (a and b) or together with pSXSRαLck F505 (c and d) were left untreated (a and c) or stimulated with pervanadate for 1 (d) or 3 min (b). Coverslips were mounted directly (a and b) or after first staining with anti-Lck (c and d). The optical sections of a complete Z series were then projected to produce the composite single (a and b) or dual (c and d) color images shown (green, ZAP-70 GFP fusion protein; red, immunostain of Lck F505). Areas of yellow represent colocalization of ZAP-70 GFP and Lck F505 (c and d).