Figure 4.
Hsp–peptide complexes reconstituted in vitro prime mice for CD8+ T cell response. Mice were immunized with HSPs alone (20–50 μg), peptides alone (10 μg), or HSP–peptide complexes (20–50 μg), as indicated. 1 wk after the last immunization, spleens were removed and stimulated with the cognate peptide or with cells transfected with the gene encoding the relevant antigen. The lymphocyte cultures were tested for their ability to lyse cells transfected with the antigen of interest (closed circle) and the nontransfected parental line (open circle). For the top panel, HSPs alone were tested for their immunizing ability in each antigenic system and were found to be consistently negative. The CTL responses were tested in many but not all systems and where tested, were found to be MHC class I and CD8 restricted.