Figure 3.
Allogeneic MLR of bone marrow–derived DCs and splenocytes. Bone marrow cells were transduced by coculture with retroviral producer lines, CreLacZ (DC–β-gal) and CreNeu (DC–Neu), and differentiated into DCs in vitro. DCs were cocultured with allogeneic, C57BL/6 T cells, isolated from bulk splenocytes by passing cells through an immunoaffinity column. After 3.5 d in culture, cells were pulsed with [3H]thymidine as described in Materials and Methods. Results from triplicate wells were corrected for [3H]thymidine incorporation by irradiated stimulators and T cells alone, and are plotted as the mean ± SEM.