Figure 7.
Effect of Rho on Fcγ receptor clustering. (A) Differential interference contrast image of a J774 cell which had been exposed to anti-Fcγ receptor antibody and to secondary antibody in the cold, followed by incubation for 5 min at 37°C to induce cross-linking of Fcγ receptors. (B) Confocal micrograph illustrating the Fcγ receptor distribution of the cell in A. Note the polar distribution of receptors. (C) Lucifer yellow emission identifying a cell which had been injected with C3 exotoxin 1 h before receptor cross-linking, which was performed as in A. (D) Confocal micrograph illustrating the Fcγ receptor distribution of the cell in C. Note that receptor clustering did not occur (compare B and D). Representative of five separate experiments of at least 20 cells/experiment.