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. 2001 Nov 12;155(4):571–580. doi: 10.1083/jcb.200104035

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Copyright © 2001, The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 2. — Comparison of Uba3 ⁺ and Uba3 ^−/− embryos in utero. Uba3 expression was analyzed by immunohistochemical stainings using anti-Uba3 antibody in Uba3 ⁺ (A) or Uba3^−/− (E) embryos at E6.0. Uba3 ⁺ (B–D) and Uba3^−/− (F–H) embryos at various stages as indicated were sagittally sectioned and stained with H&E. A cylinder-like two-layered cellular structure observed in normal embryos (B and C) was not seen in Uba3^−/− embryos (F and G). By E7.5, Uba3^−/− embryos could not be found; rather, only completely absorbed embryonic tissues could be identified in the decidua (H). EE, embryonic ectoderm; EPC, ectoplacental cone; EXE, extraembryonic ectoderm. Bars, 100 μm.