. 1996 Dec 1;184(6):2341–2352. doi: 10.1084/jem.184.6.2341

Table 1.

Immunohistochemical Characterization of Cellular Infiltrates in Ears Following Topical Challenge

Mice	Cell type^‡	T cell adoptive transfer^*		S.C. immunization^*		Nonspecific irritant^*
Mice	Cell type^‡	Sensitized	Nonsensitized	Sensitized	Nonsensitized	Sensitized	Nonsensitized
+/+	Mac/Gran	527 ± 124^§	33 ± 26	553 ± 120	19 ± 25	3,535 ± 950	100 ± 25
−/−	Mac/Gran	572 ± 142	15 ± 24	540 ± 143	18 ± 21	3,375 ± 117	236 ± 120
+/+	Gran	150 ± 95	18 ± 21	252 ± 153	18 ± 25	3,467 ± 96	335 ± 12
−/−	Gran	276 ± 141	20 ± 20	265 ± 101	17 ± 19	3,507 ± 95	184 ± 57
+/+	T cells	107 ± 59	7 ± 6	121 ± 43	25 ± 25	0	^‖
−/−	T cells	91 ± 37	12 ± 4	119 ± 41	29 ± 27	0	^‖

T cell Adoptive Transfer: purified +/+ PLN T cells from DNFB-sensitized or control mice were injected intravenously into +/+ and −/− mice. Responses were elicited with DNFB 12 h after cell injection. S.C. Immunization: DNBS-treated or control −/− spleen cells were injected subcutaneously on the dorsum +/+ and −/− mice. Responses were elicited with DNFB 5 d after cell injection. Nonspecific Irritant: 10 μl of 0.8% croton oil was painted onto the pinna of +/+ and −/− mice. Ears were harvested 24 h following DNFB challenge or 16 h following application of croton oil, embedded, and stained.

^‡

Mac/Gran: macrophages plus granulocytes, anti-Mac-1. Gran: granulocytes only, anti–GR-1. T cells, anti-Th1.2.

^§

Cells/mm².

^‖

Not done.