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. 1997 Jun 16;185(12):2043–2051. doi: 10.1084/jem.185.12.2043

Figure 7.

Figure 7

Phenotypical characterization of H-2Db−/−, β2m−/−, HHD transgenic mice. Flow cytometric analyses were performed on spleen T lymphocytes from H-2Db−/−, β2m−/−, HHD+ (left), or HHD− (right) mice. (A) Expression of H-2Kb, H-2Db, and HHD molecules detected with 20.8.4.S, B22.249.R.19, or B9.12.1 mAb, respectively and F(ab)′2 FITC-conjugated goat anti–mouse IgG, negative controls with no first mAb. Results are expressed in fluorescence intensity (x-axis, log scale) and relative cell number (y-axis). (B, top) Partial restoration of the peripheral pool of CD8+ T lymphocytes. Double staining was performed with phycoerythrin-labeled anti-CD4 (x-axis, log scale) and biotinylated anti-CD8 detected with streptavidin–Perc-P (y-axis, log scale). (Bottom) Peripheral CD8+ TCR repertoire. As illustrated for Vβ8.1.2.3, double staining was performed with FITC-labeled anti-Vβ2 (B.20.6), -Vβ3 (KJ.25), -Vβ4 (KT.10.4), -Vβ5.1,.2 (MR.9.4), -Vβ6 (44.22), -Vβ7 (TR 130), -Vβ8.1,.2,.3 (F.23.1), -Vβ9 (MR.10.2), -Vβ10 (B.21.5), -Vβ11 (RR.3.15), -Vβ13 (MR.12.4), and -Vβ17 (KJ.23.1)–specific mAb (x-axis, log scale) and phycoerythrin-labeled anti-CD8 mAb (y-axis, log scale).