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. 1997 Jun 16;185(12):2043–2051. doi: 10.1084/jem.185.12.2043

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Disruption of the H-2D^b gene. (A) Genomic structure of the H-2D^b gene. Black box, coding exon; hatched box, noncoding exons. The 3′ noncoding BamHI–HindIII probe used for Southern blot analysis and the length of the wild-type HindIII fragment are shown. (B) Restriction map of the SpeI-linearized targeting vector, which contains a 4-kb HindIII-XbaI 5′ and a 1-kb KpnI–SpeI 3′ fragment of the H-2D^b gene. (C) Predicted structure of the targeted H-2D^b gene, in which the first three exons are replaced by the whole plasmid sequence; the 1.6-kb PCRamplified diagnostic fragment and the length of the diagnostic 5.6-kb HindIII fragment generated after recombination are shown. H, HindIII; B, BamHI; X, XbaI; K, KpnI; Sp, SpeI.