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. 1997 Feb 3;185(3):541–550. doi: 10.1084/jem.185.3.541

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Influence of the E_α ^d transgenes on the number of CD4⁺ T cells. LN and thymus cell suspensions were stained with PE-labeled anti-CD4 and FITC-labeled anti-CD8 mAbs. The CD4/CD8 ratios in the LN T lymphocyte populations were determined from 5 mice per group and the following values were obtained: B6I-A^+/+, 3.03 ± 0.07; B6I-A^−/−, 0.031 ± 0.004; B6-E_α ^dI-A^−/−, 2.94 ± 0.05; B6CD11c-E_α ^dI-A^−/−, 0.04 ± 0.006. The percentages of CD4⁺CD8⁻ thymocytes in the thymi of the same animals were: B6I-A^+/+, 8.61 ± 0.71%; B6I-A^−/−, 1.06 ± 0.03%; B6-E_α ^dI-A^−/−, 6.96 ± 0.51%; B6CD11c-E_α ^dI-A^−/−, 0.77 ± 0.132%.