Table 5.
Frequency of Pluripotential and Committed Progenitors in Mice of Different Genotypes after Infection with LCMV-WE
| Genotype* | Virus | Cellularity‡ | Colonies/femur§ | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CFU-S | BFU-E | CFU-GM | CFU-Meg | |||||||||
| CD40/0 | None | 13.3 ± 1.4 | 5,978 ± 345 | 3,306 ± 500 | 33,672 ± 690 | 2,085 ± 237 | ||||||
| WE | 4.4 ± 1.1 (33%)‖ | 639 ± 163 (11%) | 566 ± 30 (17%) | 7,509 ± 899 (22%) | 312 ± 26 (15%) | |||||||
| CD80/0 | None | 13.8 ± 0.6 | 6,700 ± 379 | 3,956 ± 379 | 35,376 ± 3,790 | 2,756 ± 152 | ||||||
| WE | 3.7 ± 1.3 (27%) | 569 ± 148 (8%) | 888 ± 140 (22%) | 9,562 ± 984 (27%) | 634 ± 15 (23%) | |||||||
| P0/0 | None | 15.3 ± 1.3 | 7,452 ± 916 | 3,263 ± 33 | 27,356 ± 1,498 | 2,825 ± 162 | ||||||
| WE | 3.7 ± 0.5 (24%) | 667 ± 224 (9%) | 796 ± 111 (24%) | 7,712 ± 316 (28%) | 565 ± 56 (20%) | |||||||
| lpr | None | 19.5 ± 2.6 | ND | 3,728 ± 247 | 28,302 ± 2,860 | 2,527 ± 188 | ||||||
| WE | 7.0 ± 1.5 (35%) | ND | 559 ± 28 (15%) | 5,377 ± 524 (19%) | 430 ± 37 (17%) | |||||||
Sex-matched 10–12-wk-old C57BL/6 mice of the indicated genotypes were infected with LCMV-WE (2 × 106 PFU) or left untreated.
BM cellularity ×106/femur.
BM cells were harvested 3 d after infection and colony-forming cells per femur were determined in methylcellulose cultures containing IL-3 and erythropoietin (BFU-E), IL-3 and GM-CSF (CFU-GM), or IL-3 and thrombopoietin (CFU-Meg). CFU-S were counted on the spleen surface of lethally irradiated LCMV-immune C57BL/6 mice reconstituted with BM from infected or control mice. Shown are combined data from duplicate cultures of three mice tested individually.
Data represent means ± SD, values in brackets are relative numbers of cells/colonies compared with uninfected control mice of the same genotype.