Table 3.
BM Cytology of Wild type, IFN-α/β R0/0, and IFN-γ R0/0 Mice Infected with LCMV-WE
| Genotype | Days after infection* | Megakaryocyte§ | Cell type (%)‡ | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Erythroblast | Myeloblast promyelocyte | Myelocyte metamyelocyte band | Polymorph | Monocyte | Lymphocyte | |||||||||||
| Wild type | Uninfected | 17–25 | 35.5 ± 1 | 9.5 ± 1.8 | 36.5 ± 2.8 | 7.7 ± 1.5 | 0.8 ± 0.3 | 10 ± 1.4 | ||||||||
| Wild type | 3 | 4–11 | 15.5 ± 1.4 | 28.5 ± 1.3 | 37.5 ± 2.5 | 4.0 ± 1 | <0.5 | 14.5 ± 2.3 | ||||||||
| IFN-α/β R0/0 | 3 | 21–26 | 37.5 ± 1.5 | 10.5 ± 0.5 | 33.5 ± 2.6 | 12.0 ± 2 | 1.0 ± 0.4 | 5.5 ± 0.1 | ||||||||
| IFN-γ R0/0 | 3 | 8–13 | 17.0 ± 1.8 | 28.5 ± 1.8 | 39.5 ± 3.2 | 3.5 ± 0.5 | <0.5 | 11.5 ± 0.3 | ||||||||
*
Sex-matched mice (8–12 wk) of different genotypes (three mice per group) were infected with LCMV-WE (2 × 106 PFU). At the time points indicated, BM smears were stained with May-Günwald/Giemsa, chloracetate esterase, α-naphthyl butyrate esterase, and Sudan black B. Differential counts were obtained microscopically by counting 200 or more cells.
‡
Data are means ± SD of the relative number of total cells per lineage. Eosinophils were included in granulocyte counts.
§
Numbers represent megakaryocytes per visual field at a magnification of ×80. They were determined semiquantitatively by counting at least four visual fields.