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. 2001 Feb 19;193(4):427–434. doi: 10.1084/jem.193.4.427

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Elevated numbers of CD44^hiCD45RB^lo CD4⁺ T cells in B6.TNF^−/− mice. EAE was induced in B6.TNF^−/− and C57BL/6 mice by MOGp35–55 immunization with or without PTx coadministration, and CD44 and CD45RB phenotypes of CD4⁺ T cell were analyzed and compared with age-matched nonimmunized mice 9–11 wk later by flow cytometry. Each plot depicts CD4, CD44, and CD45RB phenotypes in total spleen cells pooled from four to five mice. Numbers underneath the regions represent the percentage of CD44^hi or CD45RB^loCD4⁺ T cells, expressed as percentage of total CD4⁺ T cells. The percentage of total CD4⁺ T cells in the spleens of the different groups did not vary significantly.