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. 2000 Jan 3;191(1):129–138. doi: 10.1084/jem.191.1.129

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Induction of IFN-γ but not IL-12 in NK1.1⁺ PECs by RMA-S cells. PECs from mice inoculated with either RMA-S or RMA-S.Ham-2 were FACS^® sorted into NK1.1⁺ and NK1.1⁻ populations and analyzed for IFN-γ (a) and IL-12 (p40) transcripts (b). Competitive PCR was used for the quantitation of transcripts (see Materials and Methods), and the intensity of the bands is displayed as percent compared with a β-actin standard. In c, the serum levels of IFN-α and IFN-β were measured using DELFIA (see Materials and Methods). Induction of IFN-γ transcription is observed in intraperitoneal NK1.1⁺ cells but not in NK1.1⁻ cells and is dependent on tumor MHC class I expression.