Figure 3.
Cytokine production from splenic T cells and serum Ig levels in response to parasite infection. (A) Splenic CD4+ T cells were prepared from uninfected animals (d0) or animals 8 d after infection (d8) with N. brasiliensis. Cells were stimulated with immobilized anti-CD3 Ab for 24 h. Supernatants were analyzed by ELISA. Data are representative of three experiments consisting of cohorts of two mice at each time point. (B) T1/ST2−/− mice (eight mice) and wild-type (six mice) were infected with N. brasiliensis. Serum IgE and IgG1 levels were measured at days 0, 8, 14, and 21 after infection. Serum samples were assayed by ELISA. Data are presented as mean ± SEM. N.D., not detectable.