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. 1999 Nov 15;190(10):1541–1548. doi: 10.1084/jem.190.10.1541

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cytokine production after in vitro Th cell differentiation. (A) CD4⁺ T cells purified from spleen from wild-type and T1/ST2^−/− mice were stimulated under Th1 or Th2 culture conditions for 5 d, washed, and restimulated on anti-CD3 Ab–coated plates for 24 h. Supernatants were analyzed for production of IL-4, IL-5, IL-10 and IFN-γ by ELISA. The experiment shown is representative of three independent experiments. (B) FACS^® analysis of intracellular cytokine. CD4⁺ T cells were cultured under Th1 or Th2 condition for 8 d, then stimulated for 4 h with PMA/ionomycin, fixed, and stained for CD4, IL-4, and IFN-γ. For analysis, gate was set on CD4⁺ lymphocytes. The frequency of cytokine-producing CD4⁺ cells is indicated in the quadrants as a percent.

Cytokine production after in vitro Th cell differentiation. (A) CD4⁺ T cells purified from spleen from wild-type and T1/ST2^−/− mice were stimulated under Th1 or Th2 culture conditions for 5 d, washed, and restimulated on anti-CD3 Ab–coated plates for 24 h. Supernatants were analyzed for production of IL-4, IL-5, IL-10 and IFN-γ by ELISA. The experiment shown is representative of three independent experiments. (B) FACS^® analysis of intracellular cytokine. CD4⁺ T cells were cultured under Th1 or Th2 condition for 8 d, then stimulated for 4 h with PMA/ionomycin, fixed, and stained for CD4, IL-4, and IFN-γ. For analysis, gate was set on CD4⁺ lymphocytes. The frequency of cytokine-producing CD4⁺ cells is indicated in the quadrants as a percent.