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. 2003 Sep 1;198(5):757–769. doi: 10.1084/jem.20030794

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Copyright © 2003, The Rockefeller University Press

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Figure 7. — LTβR^−/− mice show signs of autoimmunity. (A) The FACS^® plot shows thymic epithelial cells electronically gated on CD45-negative G8.8-positive cells. CD45⁻G8.8⁺CDR1⁻B7.1⁺ MECs were isolated by FACS^® from wild-type and LTβR^−/− mice using the indicated gate. Semi-quantitative RT-PCR results of undiluted, 5-fold, and 25-fold diluted cDNAs for several tissue-specific transcripts, HPRT, and AIRE are shown. (B) The presence of autoantibodies directed against salivary gland, pancreas, and stomach was detected in sera from LTβR^−/− but not control mice. Immunohistochemistry was performed on sections of tissues isolated from RAG2^−/− mice using 1:40 dilutions of the indicated sera. Images were obtained by confocal microscopy with identical settings for all samples shown.