Table III.
Somatic Mutations in VH186.2 Genes in FO- and MZ-derived AFC Foci and GC
| Cells sampled | Days after immunization |
Recipients (mice/group)a |
Number of sites sampledb |
VH186.2 sequences
|
Mutations/VH
(average) |
W33L mutationc |
R|Sd
|
||
|---|---|---|---|---|---|---|---|---|---|
| Total | Mutated (%) | CDR1 + 2 | FW | ||||||
| AFC foci | 8 | FO (2 mice) | ND | ||||||
| MZ (2 mice) | 1 | 12 | 0 | 0 | 0 | ||||
| 2e | 10 | 0 | 0 | 0 | |||||
| 1e | 8 | 60 | 1.2 | 0 | |||||
| 14 | FO (2 mice) | 3 | 20 | 85 | 1.5 | 12 | 10.0 | 1.7 | |
| MZ (3 mice) | 4 | 18 | 95 | 1.6 | 7 | 8.0 | 1.7 | ||
| GC | 8 | FO (2 mice) | 4 | 25 | 62 | 1.7 | 0 | ||
| MZ (2 mice) | 2 | 14 | 75 | 1.9 | 0 | ||||
| 14 | FO (2 mice) | 7 | 32 | 90 | 2.5 | 8 | 8.0 | 1.4 | |
| MZ (3 mice) | 4 | 13 | 100 | 2.8 | 0 | 11.0 | 2.1 | ||
a
Number of scid mice reconstituted with either FO B cells or MZ B cells plus TH cells and sacrificed on the indicated day after immunization with NP-CGG.
b
At least one focus (∼20–50 AFC) and one GC was microdissected from each spleen.
c
Number of sequences with tryptophan → leucine replacement in VH position 33.
d
Replacement/silent mutation ratio.
e
These foci were dissected from the same spleen.