Table II.
Expansion of Spleen Cells after Flt3L Injections
| 5 Days |
10 Days |
Fold expansion |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Control | Flt3L s.c. | Control | Flt3L s.c. | 5 Days | 10 Days | |||||
| percent | total no. ×103 | percent | total no. ×103 | percent | total no. ×103 | percent | total no. ×103 | |||
| B cells | 61 | 67,100 | 54 | 72,900 | 55 | 67,000 | 26 | 68,900 | 1.1 | 1.0 |
| T cells | 35 | 38,500 | 36 | 48,600 | 36 | 44,000 | 21 | 55,600 | 1.3 | 1.3 |
| Neutrophils | 0.9 | 990 | 1.6 | 2,160 | 0.5 | 610 | 5.9 | 15,630 | 2.2 | 25.5 |
| Monocytes | 3.3 | 3,600 | 6.1 | 8,235 | 2 | 2,400 | 9.4 | 11,470 | 2.3 | 4.8 |
| DCs | 0.6 | 660 | 2.7 | 3,645 | 0.6 | 730 | 12.6 | 15,370 | 5.5 | 21 |
| IPCs | 0.04 | 44 | 0.07 | 95 | 0.05 | 61 | 1.4 | 1,708 | 2.2 | 28 |
| CLP-DCsa | 0.1 | 106 | 0.35 | 424 | 3.9 | |||||
| CMP-DCsa | 0.17 | 176 | 0.47 | 572 | 3.2 | |||||
Changes in relative percentages and total cell number of mature hematopoietic populations in the spleen after 5 and 10 d of injections with Flt3L or PBS (control group), respectively. Results represent means of three to four animals per group and are representative of three independent experiments.
a
Percentages and total numbers of CLP- and CMP-derived DCs in spleens of transplanted animals after 10 d of Flt3L or PBS treatment, respectively. Results represent means of three experiments each involving two to four animals in the Flt3L injection and two mice in the control group.