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. 2002 Oct 21;196(8):1025–1037. doi: 10.1084/jem.20020760

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Copyright © 2002, The Rockefeller University Press

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Figure 2. — Novel ASA triggered HDHA products generated by human recombinant COX-2 ASA. 17R-HDHA. Human recombinant COX-2 treated in the presence and absence of 2 mM ASA was incubated with DHA (10 μM, 30 min, 37°C). Incubations were stopped with 2 ml cold methanol, extracted and taken for LC-MS-MS analyses. Results are representative of incubations from more than eight separate experiments, some with 1-¹⁴C-labeled DHA. (Top) LC-MS-MS chromatogram of m/z 343 showing the presence of mono-HDHA. (Bottom) MS-MS spectrum of (left) 13-HDHA without ASA treatment and (right) 17R-HDHA with ASA treatment.