Figure 3.

IL-12 production by DCs during MCMV infection. The samples used and number of experiments performed are identical to those of Fig. 1 A and C. (A) IL-12p40 and p70 titers in 24 h CM from splenic leukocytes sorted for CD11c expression. (B) IL-12p40 titers in 24 h CM from CD11c-enriched (CD11c+) versus CD11c+CD8α+-enriched (CD8α+) cell populations from day 1.5 MCMV–infected 129 RAG-2M mice. Limits of detection were 20 pg/ml for IL-12p40 and 7 pg/ml for IL-12p70. Similar results were obtained with CD8α+ cells enriched from E26 mice and with purified CD8α+Ly6G/C+ DCs from immunocompetent 129 mice (data not shown).