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. 2002 Jun 17;195(12):1647–1652. doi: 10.1084/jem.20020408

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Expression of Rel family proteins and IKKα/IKKβ/IKKγ complex in splenic B cells of PKCβ^−/− (−/−) and PKCβ^+/+ (+/+) mice. (A) The amount of RelA, RelB, and c-Rel (left panel) as well as NF-κB2 (p100 and p52, right panel) were examined by sequential Western blotting. The actin blot (left panel, bottom) was used for quantification as described in Fig. 2. For NF-κB2 both p100 and p52 are quantified in relation to p52 of +/+ lysates. The asterisk (*) indicates a nonspecific band. (B) Normal composition of the IKKα/IKKβ/IKKγ signalosome complex in PKCβ^−/− B cells. CHAPS lysates of splenic B cells from PKCβ^−/− and PKCβ^+/+ mice were immunoprecipitated with anti-IKKα antibody (IKKα) or control rabbit immunoglobulin (RIg). Whole cell lysates (WCL) are shown as blotting control. Membranes were sequentially probed with IKKα (top), IKKβ (middle), and IKKγ (bottom) antibodies.

Figure 3. — Expression of Rel family proteins and IKKα/IKKβ/IKKγ complex in splenic B cells of PKCβ^−/− (−/−) and PKCβ^+/+ (+/+) mice. (A) The amount of RelA, RelB, and c-Rel (left panel) as well as NF-κB2 (p100 and p52, right panel) were examined by sequential Western blotting. The actin blot (left panel, bottom) was used for quantification as described in Fig. 2. For NF-κB2 both p100 and p52 are quantified in relation to p52 of +/+ lysates. The asterisk (*) indicates a nonspecific band. (B) Normal composition of the IKKα/IKKβ/IKKγ signalosome complex in PKCβ^−/− B cells. CHAPS lysates of splenic B cells from PKCβ^−/− and PKCβ^+/+ mice were immunoprecipitated with anti-IKKα antibody (IKKα) or control rabbit immunoglobulin (RIg). Whole cell lysates (WCL) are shown as blotting control. Membranes were sequentially probed with IKKα (top), IKKβ (middle), and IKKγ (bottom) antibodies.