Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Oct 15;194(8):1081–1096. doi: 10.1084/jem.194.8.1081

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Compartments preloaded with a fluid-phase marker do not fuse with macropinosomes harboring L. pneumophila with intact Dot/Icm system. Bone marrow–derived macrophages were incubated at an MOI = 10 along with 1 mg/ml Rh-Dx155 for 30 min at 37°C and then incubated with bacteria either in the continued presence (A) or absence (B) of the fluid-phase marker (Materials and Methods). Number of bacteria in compartments staining with TRITC was determined as described in Material and Methods. Gray bar, LP02-GFP (intact Icm/Dot system); black bar, Lp03-GFP (dotA⁻).