Table 2.
Cell Yield from Fetal Thymus Lobe Submersion Cultures
| Culture conditions | Cell yield/well |
|---|---|
| ×10−5 | |
| No cytokine | 0.26 |
| TSLP | 0.51 |
| IL-7 | 5.9 |
| SCF | 1.1 |
| TSLP + SCF | 2.6 |
| IL-7 + SCF | 11.5 |
| TSLP + IL-7 | 3.7 |
| TSLP + IL-7+ SCF | 11.8 |
Whole day 14 fetal thymi were cultured as described (see Materials and Methods) with the indicated cytokines. After 7 d of culture, the wells were harvested and viable cell numbers were determined. The data are from a single experiment that has been performed three times with similar results.