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. 1999 Jun 7;189(11):1777–1782. doi: 10.1084/jem.189.11.1777

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MD-2 confers LPS signaling on TLR4. (A) Stable transfectants (see Materials and Methods for details) expressing TLR4 alone (▪) or TLR4 and MD-2 (•) were stimulated with LPS from E. coli 055:B5, S. minnesota Re595, or lipid A at the concentrations indicated. After a 4-h culture, cells were harvested, and luciferase activity was determined and expressed as relative light units. (B) The transfectant expressing TLR4 and MD-2 was stimulated with LPS from E. coli 055: B5, LPS from S. minnesota Re595, or lipid A at 100 ng/ml. The mAb HTA125 (black bars) or a control mAb (gray bars) were included in the indicated groups (10 μg/ml). After 4 h, NF-κB activity was determined. Data represent mean values from triplicate wells.

MD-2 confers LPS signaling on TLR4. (A) Stable transfectants (see Materials and Methods for details) expressing TLR4 alone (▪) or TLR4 and MD-2 (•) were stimulated with LPS from E. coli 055:B5, S. minnesota Re595, or lipid A at the concentrations indicated. After a 4-h culture, cells were harvested, and luciferase activity was determined and expressed as relative light units. (B) The transfectant expressing TLR4 and MD-2 was stimulated with LPS from E. coli 055: B5, LPS from S. minnesota Re595, or lipid A at 100 ng/ml. The mAb HTA125 (black bars) or a control mAb (gray bars) were included in the indicated groups (10 μg/ml). After 4 h, NF-κB activity was determined. Data represent mean values from triplicate wells.