Figure 3.
Membrane anchoring of MD-2 via TLR4. A stable line expressing MD-2 with the flag epitope was stained with the anti-flag mAb, followed by goat anti–mouse IgG–FITC (a and d). Staining with cell permeabilization is shown in d. Another stable transfectant was used that expressed MD-2 with the protein C epitope and TLR4 in b, c, and e. Cell surface MD-2 and TLR4 are shown with the rat anti–protein C mAb or the mouse anti–human TLR4 mAb HTA125 (b and c, respectively). MD-2 expression in permeabilized cells is shown in e. The second reagents used were goat anti–rat IgG–PE or goat anti–mouse IgG–FITC, respectively. Control histograms stained with isotype-matched mouse or rat mAb are shown (dotted lines).