Table I.
Analysis of SRCs after 2, 4, and 6 d of Ex Vivo Culture in the Presence of BMPs
| Treatments | Day 2 | Day 4 | Day 6 | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. pos | No. neg | % positive | No. pos | No. neg | % positive | No. pos | No. neg | % positive | ||||||||||
| Control SF | 7 | 0 | 100 | 10 | 1 | 100 | 0 | 9 | 0 | |||||||||
| α–TGF-β (50 ng/ml) | 1 | 1 | 50 | 2 | 0 | 100 | 1 | 3 | 25 | |||||||||
| TGF-β (400 pM) | 3 | 0 | 100 | 3 | 7 | 30 | 0 | 2 | 0 | |||||||||
| BMP-2 (5 ng/ml) | 7 | 0 | 100 | 6 | 0 | 100 | 1 | 3 | 25 | |||||||||
| BMP-2 (50 ng/ml) | ND | 0 | 4 | 4 | ND | |||||||||||||
| BMP-7 (5 ng/ml) | 6 | 0 | 100 | 7 | 0 | 100 | 0 | 5 | 0 | |||||||||
| BMP-7 (50 ng/ml) | ND | 1 | 3 | 25 | 0 | 4 | 0 | |||||||||||
| BMP-4 (5 ng/ml) | ND | 1 | 2 | 33 | 0 | 4 | 0 | |||||||||||
| BMP-4 (25 ng/ml) | 1 | 0 | 100 | 7 | 0 | 100 | 5 | 1 | 83 | |||||||||
| 5% FCS | 0 | 4 | 0 | 0 | 3 | 0 | ND | |||||||||||
Summary of the frequency of engrafted mice after transplantation of CD34+CD38−Lin− cells after ex vivo culture. The human cell engraftment in the BM of 121 mice transplanted with CD34+CD38−Lin− cells expanded for 2, 4, and 6 d was determined by DNA extraction 8 wk after transplant and hybridized with a human chromosome 17–specific α-satellite probe as shown previously. The percentage of human engraftment in the BM of positive mice ranged between 0.1 and 1%. pos, positive; neg, negative.