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Synergistic effect of α-GalCer and IL-12 in vivo. C57BL/6 mice were injected with a suboptimal dose of α-GalCer (200 ng/mouse i.v.) and 6 h later, mice were injected with a suboptimal dose of IL-12 (200 U/mouse i.p.). 1 d after the treatment with IL-12, the mice were killed and splenic natural killing activity (A) and serum IFN-γ levels (B) were determined as described in Materials and Methods. The bars represent mean ± SE of triplicate samples.
