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. 1999 Jan 18;189(2):413–422. doi: 10.1084/jem.189.2.413

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(a) Tissue distribution of AIM gene expression. 10 μg of total RNA from various mouse (B6) tissues and from adherent PECs induced by thioglycollate from RAG-2^−/− mouse was hybridized with either AIM cDNA or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA fragment. Weak background signals derived from 18S rRNA, which is slightly shorter than AIM rRNA, can be detected in every tissue. In this experiment, adherent PECs were prepared from RAG-2^−/− mice to avoid any contamination of T and B (B-1 and B-2) cells. However, almost comparable expression was detected in the adherent PECs from wild-type B6 mice (data not shown). (b–i) In situ hybridization analysis of AIM expression (violet/dark blue signals in b, d, f, and h) and its comparison with immunolocalization of pan-macrophage antigen, F4/80 (dark brown signals in c, e, g, and i), in mouse granulomas in the liver (b and c), thymus (d and e), spleen (f and g), and liver (h and i). (b and c) AIM is expressed in a subset of macrophages in the peripheral areas of granulomas (gp) but not in the central areas (gc). g, granulomas; lv, liver tissue. (d and e) AIM is expressed by a subpopulation of cortical macrophages as indicated by arrows (d), although macrophages exist in both cortex and medulla (e). c, cortex; m, medulla. (f and g) AIM is expressed predominantly by a subset of macrophages in the marginal zones. r, red pulp; w, white pulp; mz, marginal zone. (h and i) AIM is expressed by a subpopulation of Kupffer/macrophage cells. Original magnifications: b, ×400; c, e, g, h, and i, ×100; d and f, ×200. (j) No AIM expression was detected in cultured macrophages. Adherent PECs were prepared from intraperitoneal thioglycollate-injected RAG-2^−/− mice. A portion of the cells were cultured on plastic dishes for 16 h in complete DMEM containing 10% FCS. Total RNA was prepared from freshly isolated and cultured macrophages, and hybridized with either AIM or GAPDH cDNA fragment.

(a) Tissue distribution of AIM gene expression. 10 μg of total RNA from various mouse (B6) tissues and from adherent PECs induced by thioglycollate from RAG-2^−/− mouse was hybridized with either AIM cDNA or glyceraldehyde-3-phosphate dehydrogenase (GAPDH) cDNA fragment. Weak background signals derived from 18S rRNA, which is slightly shorter than AIM rRNA, can be detected in every tissue. In this experiment, adherent PECs were prepared from RAG-2^−/− mice to avoid any contamination of T and B (B-1 and B-2) cells. However, almost comparable expression was detected in the adherent PECs from wild-type B6 mice (data not shown). (b–i) In situ hybridization analysis of AIM expression (violet/dark blue signals in b, d, f, and h) and its comparison with immunolocalization of pan-macrophage antigen, F4/80 (dark brown signals in c, e, g, and i), in mouse granulomas in the liver (b and c), thymus (d and e), spleen (f and g), and liver (h and i). (b and c) AIM is expressed in a subset of macrophages in the peripheral areas of granulomas (gp) but not in the central areas (gc). g, granulomas; lv, liver tissue. (d and e) AIM is expressed by a subpopulation of cortical macrophages as indicated by arrows (d), although macrophages exist in both cortex and medulla (e). c, cortex; m, medulla. (f and g) AIM is expressed predominantly by a subset of macrophages in the marginal zones. r, red pulp; w, white pulp; mz, marginal zone. (h and i) AIM is expressed by a subpopulation of Kupffer/macrophage cells. Original magnifications: b, ×400; c, e, g, h, and i, ×100; d and f, ×200. (j) No AIM expression was detected in cultured macrophages. Adherent PECs were prepared from intraperitoneal thioglycollate-injected RAG-2^−/− mice. A portion of the cells were cultured on plastic dishes for 16 h in complete DMEM containing 10% FCS. Total RNA was prepared from freshly isolated and cultured macrophages, and hybridized with either AIM or GAPDH cDNA fragment.