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. 1976 Nov 2;144(5):1214–1226. doi: 10.1084/jem.144.5.1214

Regulatory mechanisms in cell-mediated immune responses. IV. Expression of a receptor for mixed lymphocyte reaction suppressor factor on activated T lymphocytes

PMCID: PMC2190455  PMID: 1086882

Abstract

Suppression of the mixed lymphocyte reaction (MLR) by a soluble factor produced by alloantigen-activated spleen cells requires genetic homology between the factor-producing cells and responder cells in MLR. The ability of lymphocytes used as MLR responder cells to adsorb MLR suppressor factor was tested to investigate the expression of a receptor structure for suppressor molecules. Normal spleen or thymus cells had no effect on suppressor activity. Concanavalin A (Con A)- activated thymocytes, however, effectively removed suppressor activity, suggesting that the receptor is expressed only after activation and is not present or not functional on resting cells. Significantly neither phytohemagglutinin- nor lipopolysaccharide-activated lymphoid cells absorbed the factor. Furthermore, only Con A-activated thymocytes demonstrating genetic homology with the cell producing suppressor factor for H-2 regions to the right of I-E were effective absorbants. Alloantigen-stimulated spleen cells syngeneic to the suppressor cell also removed suppressor activity. These data support an hypothesis that subsequent to stimulation in MLR, T lymphocytes express a receptor, either through synthesis or alteration of an existing molecular structure, which then provides the appropriate site for interaction with suppressor molecules.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson C. L., Grey H. M. Receptors for aggregated IgG on mouse lymphocytes: their presence on thymocytes, thymus-derived, and bone marrow-derived lymphocytes. J Exp Med. 1974 May 1;139(5):1175–1188. doi: 10.1084/jem.139.5.1175. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cullen S. E., David C. S., Shreffler D. C., Nathenson S. G. Membrane molecules determined by the H-2 associated immune response region: isolation and some properties. Proc Natl Acad Sci U S A. 1974 Mar;71(3):648–652. doi: 10.1073/pnas.71.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. David C., Meo T., McCormick J., Shreffler D. Expression of individual Ia specificities on T and B cells. I. Studies with mitogen-induced blast cells. J Exp Med. 1976 Jan 1;143(1):218–224. doi: 10.1084/jem.143.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Edelman G. M. Surface modulation in cell recognition and cell growth. Science. 1976 Apr 16;192(4236):218–226. doi: 10.1126/science.769162. [DOI] [PubMed] [Google Scholar]
  5. Gershon R. K. A disquisition on suppressor T cells. Transplant Rev. 1975;26:170–185. doi: 10.1111/j.1600-065x.1975.tb00179.x. [DOI] [PubMed] [Google Scholar]
  6. Harrison M. R., Paul W. E. Stimulus-response in the mixed lymphocyte reaction. J Exp Med. 1973 Dec 1;138(6):1602–1607. doi: 10.1084/jem.138.6.1602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hauptfeld M., Hauptfeld V., Klein J. Ia and H-2 antigens on blast cells. Transplantation. 1975 Jun;19(6):528–530. [PubMed] [Google Scholar]
  8. Howe M. L., Cohen L. Lymphoid cell subpopulations. II. Characterization of cell populations responsible for syngery in the mixed lymphocyte interaction. J Immunol. 1975 Nov;115(5):1233–1238. [PubMed] [Google Scholar]
  9. Howe M. L., Manziello B. Ontogenesis of the in vitro response of murine lymphoid cells to cellular antigens and phytomitogens. J Immunol. 1972 Sep;109(3):534–539. [PubMed] [Google Scholar]
  10. Hudson L., Sprent J. Specific adsorption of IgM antibody onto H-2-activated mouse T lymphocytes. J Exp Med. 1976 Feb 1;143(2):444–449. doi: 10.1084/jem.143.2.444. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Jandinski J., Cantor H., Tadakuma T., Peavy D. L., Pierce C. W. Separation of helper T cells from suppressor T cells expressing different Ly components. I. Polyclonal activation: suppressor and helper activities are inherent properties of distinct T-cell subclasses. J Exp Med. 1976 Jun 1;143(6):1382–1390. doi: 10.1084/jem.143.6.1382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Krammer P. H., Hudson L., Sprent J. Fc-receptors, Ia-antigens, and immunoglobulin on normal and activated mouse T lymphocytes. J Exp Med. 1975 Dec 1;142(6):1403–1415. doi: 10.1084/jem.142.6.1403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lonai P., McDevitt H. O. I-region genes are expressed on T and B lymphocytes. Studies of the mixed lymphocyte reaction (MLR). J Exp Med. 1974 Nov 1;140(5):1317–1323. doi: 10.1084/jem.140.5.1317. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Munro A. J., Taussig M. J. Two genes in the major histocompatibility complex control immune response. Nature. 1975 Jul 10;256(5513):103–106. doi: 10.1038/256103a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Nabholz M., Young H., Rynbeek A., Boccardo R., David C. S., Meo T., Miggiano V., Shreffler D. C. I-region-associated determinants: expression on mitogen-stimulated lymphocytes and detection by cytotoxic T cells. Eur J Immunol. 1975 Sep;5(9):594–599. doi: 10.1002/eji.1830050903. [DOI] [PubMed] [Google Scholar]
  17. Nagy Z., Elliott B. E., Nabholz M., Krammer P. H., Pernis B. Specific binding of alloantigens to T cells activated in the mixed lymphocyte reaction. J Exp Med. 1976 Mar 1;143(3):648–659. doi: 10.1084/jem.143.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pierce C. W. Immune responses in vitro. I. Cellular requirements for the immune response by nonprimed and primed spleen cells in vitro. J Exp Med. 1969 Aug 1;130(2):345–364. doi: 10.1084/jem.130.2.345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Rich R. R., Pierce C. W. Biological expressions of lymphocyte activation. 3. Suppression of plaque-forming cell responses in vitro by supernatant fluids from concanavalin A-activated spleen cell cultures. J Immunol. 1974 Apr;112(4):1360–1368. [PubMed] [Google Scholar]
  20. Rich S. S., Rich R. R. Regulatory mechanisms in cell-mediated immune responses. I. Regulation of mixed lymphocyte reactions by alloantigen-activated thymus-derived lymphocytes. J Exp Med. 1974 Dec 1;140(6):1588–1603. doi: 10.1084/jem.140.6.1588. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rich S. S., Rich R. R. Regulatory mechanisms in cell-mediated immune responses. II. A genetically restricted suppressor of mixed lymphocyte reactions released by alloantigen-activated spleen cells. J Exp Med. 1975 Dec 1;142(6):1391–1402. doi: 10.1084/jem.142.6.1391. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rich S. S., Rich R. R. Regulatory mechanisms in cell-mediated immune responses. III. I-region control of suppressor cell interaction with responder cells in mixed lymphocyte reactions. J Exp Med. 1976 Mar 1;143(3):672–677. doi: 10.1084/jem.143.3.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Shreffler D. C., David C. S. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization. Adv Immunol. 1975;20:125–195. doi: 10.1016/s0065-2776(08)60208-4. [DOI] [PubMed] [Google Scholar]
  24. Stobo J. D. Phytohemagglutin and concanavalin A: probes for murine 'T' cell activivation and differentiation. Transplant Rev. 1972;11:60–86. doi: 10.1111/j.1600-065x.1972.tb00046.x. [DOI] [PubMed] [Google Scholar]
  25. Stout R. D., Herzenberg L. A. The Fc receptor on thymus-derived lymphocytes: II. Mitogen responsiveness of T lymphocytes bearing the Fc receptor. J Exp Med. 1975 Nov 1;142(5):1041–1051. doi: 10.1084/jem.142.5.1041. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taniguchi M., Hayakawa K., Tada T. Properties of antigen-specific suppressive T cell factor in the regulation of antibody response of the mouse. II. In vitro activity and evidence for the I region gene product. J Immunol. 1976 Feb;116(2):542–548. [PubMed] [Google Scholar]
  27. Yoshida T. O., Andersson B. Evidence for a receptor recognizing antigen complexed immunoglobulin on the surface of activated mouse thymus lymphocytes. Scand J Immunol. 1972;1(4):401–408. doi: 10.1111/j.1365-3083.1972.tb03306.x. [DOI] [PubMed] [Google Scholar]
  28. von Boechmer H. Separation of T and B lymphocytes and their role in the mixed lymphocyte reaction. J Immunol. 1974 Jan;112(1):70–78. [PubMed] [Google Scholar]

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