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. 2000 Oct 2;151(1):41–52. doi: 10.1083/jcb.151.1.41

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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LN-agrin accounts for all agrin in BLs. Immunohistochemistry with an anti-agrin antibody shows that agrin is present in BLs of cerebral microvasulature (A), renal glomeruli (C), and pia and sheath of the optic nerve (E) at E18. No immunoreactivity is detectable at any of these sites in agrin^LN/LN mice (B, D, and F). Basal laminae remain intact as indicated by anti-laminin staining in wild-type (G) and agrin^LN/LN mutant (H) kidney. Bar: (A, B, E, and F) 250 μm; (C and D) 50 μm; and (G and H) 125 μm.