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. 2000 Oct 2;151(1):41–52. doi: 10.1083/jcb.151.1.41

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The NH₂ terminus of SN-agrin mediates its association with cells. CHO cells were transfected with vectors encoding the NH₂ terminus of SN-agrin fused to FLAG (A and B), full-length SN-agrin (C and D), or the NH₂ terminus of LN-agrin fused to FLAG (E and F). Cells were stained with anti-FLAG (A, B, E, and F) or anti-agrin (C and D), either live to detect cell surface proteins (A, C, and E) or after fixation and permeabilization (B, D, and F). SN-agrin reached the cell surface, but no staining of live cells was detctable with the LN–FLAG construct. Staining of fixed and permeabilized cells indicated expression levels were similar for each construct. Bar, 20 μm.