Fig. 3.
CAF-1-depleted cells assemble normal replication foci and accumulate in early to mid S-phase. (A) Cells were stained for chromatin-bound PCNA and scored for replication patterns. Examples of early, mid, and late S-phase patterns are provided. (B) Late S-phase patterns are easily seen in control cells but are found less frequently in cells treated with either CAF-1-targeted siRNA. Arrows indicate examples of early, middle, and late replication sites (▹, early S-phase patterns; →, middle S-phase patterns; ▸, late S-phase patterns). (C) Distribution of S-phase cells displaying early, mid, or late S-phase replication patterns 48 h after transfection. Three hundred S-phase cells were counted per sample, and the results are the mean from two independent experiments.