Figure 2.
Calculation of excitation ratios rex,i: Fluorescence reference spectra of CFP (numerator of Eq. 3) are fitted to YFP reference spectra (denominator of Eq. 3), resulting in a scaling factor rex,1 = 2.29 for data obtained using excitation wavelength λ2 = 458 nm (A). The same procedure applied to data with excitation wavelength λ2 = 488 nm result in rex,2 = 0.02 (B). The two lower panels in both panels A and B show the residuals of the fits and the ratios rex,i as functions of emission wavelength.