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. 2000 Oct 30;151(3):587–600. doi: 10.1083/jcb.151.3.587

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Properties of the NH₂-terminal domain of Pep12p. (A) GFP-tagged PNTP and the F20L mutant version both reach vacuoles in wild-type cells. PNTS, the equivalent construct with the Sso1 TMD, goes to vacuoles in wild-type and end4 cells, whereas the F20L mutant goes to the plasma membrane. (B) GFP-labeled derivatives of Ste18p (as diagrammed) were expressed in a Δste18 strain and imaged. Equivalent constructs without GFP, or no construct (−), were expressed in Δste18 cells carrying a FUS1-lacZ reporter and the cells assayed for β-galactosidase activity. Note the log scale of the graph.