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. 2000 Oct 30;151(3):587–600. doi: 10.1083/jcb.151.3.587

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Targeted mutagenesis of the sorting signal. (A) Images of the parental construct (GFP-tagged Pep12-Sso1) and missorted mutants in wild type and end4 strains. (B) Sequence changes in constructs that were missorted or showed a normal location. Dashes in the NM38 sequence indicate a deletion. *Mutants shown in A. (C) Distribution in end4 cells of a construct containing only residues 2–44 of Pep12p (structure as diagrammed), or the M18 and M83 mutant versions (see Fig. 1).