Figure 4.
Localization of Prm1p. (A–C) A strain of mating type a bearing a PRM1-GFP fusion gene was treated with 10 μg/ml α factor. Samples were taken and imaged on a confocal microscope after 40, 70, and 100 min of incubation, respectively. Apparent loss of ER staining in B and C is due primarily to differences in signal gain used to collect each image. (D–F) Strains of opposite mating types, each bearing the PRM1-GFP fusion gene, were mixed, concentrated, and spotted on a YPD plate. After ∼2 h at 30°C, cells were resuspended and imaged as above. Images of representative cells are shown.