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. 2000 Aug 21;150(4):839–848. doi: 10.1083/jcb.150.4.839

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Physiological stimulation induced compound exocytosis in lactotrophs. (a) Lactotrophs were bathed in a solution containing 4 μM FM1-43. Only the cell's surface membrane was initially stained with dye. Images of an individual lactotroph were obtained before (left) and after (right) wash-in of 200 nM TRH. Bright fluorescent spots were induced by stimulation. (b) Fluorescent spots appeared on two overlapping cells that were stimulated with 500 nM VIP in the presence of 4 μM FM1-43. The figure shows the cell before (left) and after (right) stimulation. (c) Histogram of normalized spot brightness measured during stimulation with VIP. The spots were not different than those induced by high K⁺ stimulation in the number of spots/equator, the brightness of the spots, or the percentage of events that was compound (Table ). The smooth line represents the scaled unitary fluorescence values from Fig. 2 d.

Physiological stimulation induced compound exocytosis in lactotrophs. (a) Lactotrophs were bathed in a solution containing 4 μM FM1-43. Only the cell's surface membrane was initially stained with dye. Images of an individual lactotroph were obtained before (left) and after (right) wash-in of 200 nM TRH. Bright fluorescent spots were induced by stimulation. (b) Fluorescent spots appeared on two overlapping cells that were stimulated with 500 nM VIP in the presence of 4 μM FM1-43. The figure shows the cell before (left) and after (right) stimulation. (c) Histogram of normalized spot brightness measured during stimulation with VIP. The spots were not different than those induced by high K⁺ stimulation in the number of spots/equator, the brightness of the spots, or the percentage of events that was compound (Table ). The smooth line represents the scaled unitary fluorescence values from Fig. 2 d.