Figure 12.
Microinjection of Δ17ARF1 inhibits the LPA-induced assembly of paxillin-containing focal adhesions. Serum-starved Swiss 3T3 cells were microinjected with buffer alone (A and B) or Δ17ARF1 (C–F) at a needle concentration of 900 μg/ml. After 20 min, the cells were stimulated with 1 μg/ml LPA for 15 min, fixed and stained for either paxillin, by indirect immunofluorescence (A, C, and E), or F-actin with FITC-phalloidin (B, D, and F). Microinjected cells are indicated by arrowheads. The nuclear and cell body staining in the paxillin channel is bleed-through from the TRITC-Dextran used to aid identification of the microinjected cells. The F-actin staining has been deliberately overexposed to show the weaker actin fibers in cells microinjected with Δ17ARF1. Bar, 10 μm.