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. 2000 Apr 3;149(1):181–194. doi: 10.1083/jcb.149.1.181

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Scanning EM analysis of the interaction between Jurkat T cell and anti-CD3–coated beads. Untransfected Jurkat T cells (a), T cells transfected with GFP-ActA repeats (b), and T cells transfected with GFP-ActA repeats F > A (c) were incubated with anti-CD3–coated beads, fixed, and processed for scanning EM. Jurkat T cells, which expressed GFP-ActA repeats, formed a very loose contact with the bead. In contrast, control T cells or cells which expressed the mutated GFP-ActA repeats extended sheet-like protrusions that almost completely surrounded the beads. Bar, 5 μm.