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. 2000 May 1;149(3):647–656. doi: 10.1083/jcb.149.3.647

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© 2000 The Rockefeller University Press

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Relative distributions of β spectrin and ankyrin in copper cells. (A) Copper cell morphology. Copper cells alternate with interstitial cells in the larval middle midgut epithelium. The apical surface of the copper cell is invaginated and opens into the gut lumen through a pore formed by surrounding interstitial cells. The basolateral surface is spheroid in shape and completely surrounds the invaginated apical domain, except at the pore. The basolateral domain immediately surrounding the pore (apicolateral, arrow) forms a septate junction with the adjacent region of the interstitial cells. (B) Dissected middle midgut from a first instar larva expressing the myc epitope–tagged β spectrin transgene stained with mouse mAb against the epitope tag and Texas red–labeled goat anti–mouse secondary antibody. The most intense staining was found at the apicolateral domain of copper cells (arrowhead), with weaker staining of the rest of the basolateral plasma membrane (arrow). (C) Double labeling of the same sample as in A, stained with affinity-purified rabbit anti-ankyrin antibody and FITC-conjugated secondary antibody. Arrowhead marks comma-shaped staining of the apicolateral contacts between copper cells and neighboring interstitial cells in this optical section. (D) Staining of a second instar β-spec ⁺ larva with ankyrin antibody as in C. Arrow marks staining of the basolateral domain of copper cells and arrowhead marks the relatively intense staining of the apicolateral contact region. Bar, 10 μM.

Relative distributions of β spectrin and ankyrin in copper cells. (A) Copper cell morphology. Copper cells alternate with interstitial cells in the larval middle midgut epithelium. The apical surface of the copper cell is invaginated and opens into the gut lumen through a pore formed by surrounding interstitial cells. The basolateral surface is spheroid in shape and completely surrounds the invaginated apical domain, except at the pore. The basolateral domain immediately surrounding the pore (apicolateral, arrow) forms a septate junction with the adjacent region of the interstitial cells. (B) Dissected middle midgut from a first instar larva expressing the myc epitope–tagged β spectrin transgene stained with mouse mAb against the epitope tag and Texas red–labeled goat anti–mouse secondary antibody. The most intense staining was found at the apicolateral domain of copper cells (arrowhead), with weaker staining of the rest of the basolateral plasma membrane (arrow). (C) Double labeling of the same sample as in A, stained with affinity-purified rabbit anti-ankyrin antibody and FITC-conjugated secondary antibody. Arrowhead marks comma-shaped staining of the apicolateral contacts between copper cells and neighboring interstitial cells in this optical section. (D) Staining of a second instar β-spec ⁺ larva with ankyrin antibody as in C. Arrow marks staining of the basolateral domain of copper cells and arrowhead marks the relatively intense staining of the apicolateral contact region. Bar, 10 μM.