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SNAREpins are resistant to a challenge with αSNAP and NSF and BoNT D light chain. Standard amounts of v- and t-SNARE vesicles were incubated overnight at 4°C. The incubation was then continued at 37°C in the presence of buffer (open diamonds), BoNT D–light chain (open circles) or BoNT D–light chain and αSNAP, NSF, and Mg-ATP (closed circles). Fusion was followed and normalized as described in Fig. 1 a.
