Figure 3.
αSNAP and NSF can disrupt bona fide cis-SNARE complexes. The NH2 terminus of syntaxin was removed by thrombin treatment of t-SNARE liposomes containing a thrombin-cleavable version of syntaxin as described (Parlati et al. 1999). v- and t-SNARE vesicles lacking the NH2-terminal domain of syntaxin (Parlati et al. 1999) were incubated at 37°C in the presence of cytosolic domain VAMP and in the presence of buffer (open diamonds), αSNAP, NSF, and Mg-ATP (closed circles), or αSNAP, NSF, and EDTA-ATP (open circles). Fusion was followed and normalized as described in Fig. 1 a.