Table 2.
Number of Asters per Blastomere in the Injected Half of Embryos
| Number of asters/blastomere | ||||||
|---|---|---|---|---|---|---|
| Injection | 1 | 2 | 3 | 4 | 5 or more | Blastomeres |
| % | % | % | % | % | n | |
| HsCen3p | 5 | 91 | 1 | 3 | 0 | 103 |
| WT HsCEN3 RNA | 10 | 78 | 3 | 3.5 | 5.5 | 111 |
| Mutant HsCEN3 RNA | 18 | 68.5 | 3 | 2.5 | 8 | 126 |
7 h (protein injection) or 11 h (RNA injection) after fertilization, embryos were fixed and labeled with monoclonal anti-α–tubulin. For each condition, the number of asters per large blastomere in the injected half of the embryo was scored in two embryos from two independent experiments. One or two asters were observed in blastomeres cleaved at normal rate.