Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Mar 20;148(6):1255–1266. doi: 10.1083/jcb.148.6.1255

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Schematic representation of the functional domain of KAP3 and the α-fodrin molecule obtained by the yeast two-hybrid system. This scheme depicts the entire amino acid sequence of the KAP3 molecule (1–793). We constructed bait vectors of various lengths. The minimal required domain for interaction with fodrin consisted of aa 209–292, as indicated. Determination of the binding affinity using liquid yeast cultures was also performed, and the results are summarized at the right of the figure. The minimal required domain (209–291) showed strongest binding affinity (indicated by ++).