Figure 3.
Export of CTA1 from microsomes. (a) After translation (10 min) of CTA1 in a single reaction, the microsomes were reisolated by centrifugation, resuspended in PBS, and divided into aliquots. The export reaction was started by the addition of export buffer with or without ATP. At the end of the chase period the microsomes were reisolated by centrifugation. The presence of CTA1 in the microsomal pellet and the supernatant was analyzed by SDS-PAGE and autoradiography. (b) Statistical analysis of export reactions performed for 1 h without ATP (n = 5), with ATP (n = 13), or with ATP and cytosol (n = 5). For the calculation of export rates, the fraction of CTA1 found in the supernatant at 0 min chase was subtracted. Bars represent SD.