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. 2000 Mar 20;148(6):1305–1315. doi: 10.1083/jcb.148.6.1305

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Dose dependence and time-course analysis of bacterially induced upregulation of matrilysin. (A) HT29 human colon epithelial cells were infected for 90 min with E. coli strain ORN103/pSH2 (type 1⁺/fimH ⁺) at the indicated bacterial/epithelial cell ratio. (B) HT29 cells were infected with the recombinant strain ORN103/pSH2, at a ratio of 300 bacteria per epithelial cell, for different periods of time, from 5 to 60 min. conditioned medium samples were collected at 48 h after infection and analyzed by Western blotting. (Pro) 28-kD proform of matrilysin; and (Mat) 19-kD activated matrilysin.