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. 2002 Jan 7;156(1):29–34. doi: 10.1083/jcb.200111004

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — Functional characterisation of Yvc1p. (a) Yvc1–GFP localization to the vacuolar membrane, as visualized by fluorescence microscopy using an FITC filter. (b) YVC1 overexpression causes Ca²⁺ sensitivity, as shown by serial fivefold dilutions of wild-type (YPH499) strain transformed with a control (CTL) or the pYVC1-L-HA plasmid allowing high expression levels. Because YVC1-expressing cells grew slightly more slowly than control cells on regular media (unpublished data), low Ca²⁺ SD medium (see Materials and methods) was used to perform this experiment in order to have identical growth on the control plate. (c) YVC1 is required for vacuolar Ca²⁺ release in response to hypertonic shock. Luminescence response of the wild-type strain (YPH499), carrying pYVC1-U for YVC1 overexpression (WT+YVC1o.p.) or a control plasmid (WT), and of the yvc1Δ strain (VDY23) carrying a control plasmid (yvc1Δ). (d) Same experiment as c in pmc1Δvcx1Δ (KKY124) and pmc1Δvcx1Δyvc1Δ (VDY40) strains. (e) Same experiment as c in vcx1Δ (KKY127) and vcx1Δyvc1Δ (VDY31) strains.