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. 2003 Oct 27;163(2):271–282. doi: 10.1083/jcb.200306134

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Copyright © 2003, The Rockefeller University Press

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Figure 3. — Effects of rapid applications of IP₃ and cADPR to isolated nuclei loaded with Mag-Fura Red™. (A) Arrangement for experiments with uncaging of IP₃ inside the nucleus (see Materials and methods). The colored boxes correspond to the colored traces in B–E, representing the Ca²⁺ concentrations in the indicated parts of the nuclear envelope, during uncaging of IP₃ in the center of the nucleus (UV). (F) Effects of ionophoretic IP₃ application. (G) Effect of ionophoretic cADPR application. (H) Bleaching-recovery experiment in isolated nucleus. Bleaching by laser light at 488 nm caused marked reduction in fluorescence intensity in affected area followed by fluorescence recovery due to diffusion from nonbleached areas. (I) Simulated time course of the open probability of (a) type 2 and (b) type 3 IP₃ receptors in response to 20 μM IP₃. (J) Simulation of the release and reuptake of Ca²⁺ in the nuclear envelope in response to IP₃ stimulation. [IP₃] = 20/(1 + e^{(−(t−10)/2)}) (μM) in this simulation, to model the gradual increase of IP₃ by perfusion (for further details see supplemental information, available at http://www.jcb.org/cgi/content/full/jcb.200306134/DC1).