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PCR amplification of the delivered DNA in various subcellular fractions of hepatocytes. Total DNA from various subcellular fractions of parenchymal cells was analyzed by PCR using CAT primers. E, experimental; HC, heat control; FD, free DNA. The lanes are: N, nuclear; C, cytosolic; and L, lysosomal/mitochondrial. The 633-bp CAT gene fragment amplified from pCIS3CAT was used as a control (+).
